I prepared, separately, rabbit anti-rat P4HB (1:50 dilution) and rabbit anti-rat CD68 (1:100 dilution) primary antibodies in 10% normal goat serum (in TTBS w/ 0.05% Triton-X 100, as per our usual protocol). I cannot process the IHC slides on the same day, due to unforeseen circumstances. Are my diluted antibodies stable in the refrigerator for a day or two? The stock CD68 antibody should be stored in the fridge anyway, but the stock P4HB antibody needs to be in the freezer.
Dear Laura,
Your primary antibodies will be OK in the fridge for many days or several weeks.
Best Wishes, Refik
Dear Laura,
Your primary antibodies will be OK in the fridge for many days or several weeks.
Best Wishes, Refik
It will probably work, the storage indicated is for a long term storage, for few days place it at 4°C
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