Does anybody have any idea whether fixation protocol for IHC affects the post translational modification of antigens? Thank you in advance!
What type of PTM are you looking at? And just to be clear, are you looking at formalin-fixed, parafin-embedded samples? A little more info would help us with your problem. That being said, PBS can often occlude phosphates. As a result, people will often use TBS instead. Similarly, milk can occlude also phosphates, particularly on Tyrosine. As a result, people will often use serum or BSA to block and dilute antibodies. As for fixation itself? Hard to say. Most use formalin-embedded tissues for IHC or fresh frozen. You can also certainly have problems with antigen retrieval of formalin-fixed, parafin-embedded samples.
What type of post transnational modification are you looking for? is it physical, chemical or functional? not clear.And my question for you is how is post-translational modification possible in a fixed tissue?
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