Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Which variables can be used to increase/decrease the grain size of czochralski-grown polycrystalline silicon?

Which variables (such as pull speed, melt temperature, annealing temperature/duration, cooling rate, etc.) affect the grain size of czochralski-grown polycrystalline silicon? In what direction...

10 January 2021 2,204 6 View

How do I justify the use of fish scales to synthesize a nano filter?

Good day. I am doing a studu on the use of fish scales to create a nano filter. This is aimed at potentially solving the face mask problem brought about the pandemic. This is what I know so...

09 January 2021 8,907 5 View

Calculating Intercoder Reliability for 3 coders with 7 categories across 6 texts?

How would you calculate inter-coder reliability (Kappa coefficient?) for the following scenario: 6 texts coded across 7 categories (codes) by 3 coders?

23 December 2020 2,324 3 View

No title

10 October 2020 3,407 2 View

Do you agree that one of the advantages of the Boundary Element Method is that it easily addresses Incompressible Materials (Poisson's Ratio = 0.50)?

When modeling incompressible materials via the Finite Element Method (FEM), typically the plane formulation that is displacement based fails when Poission's Ratio = 0.50, which is a standard...

08 October 2020 7,058 1 View

Recommended analysis for an ANCOVA-like problem with binary variables for dependent variable and covariate?

What is your recommended analysis for the following problem: I have 3 (treatment) groups and a non-randomized pre-post design. I want analyze the effects of the groups on the follow-up measure,...

28 September 2020 5,890 4 View

No title

18 August 2020 3,955 11 View

Are there any test methods for butterflies or other arthropod pollinator besides bees?

In the current EFSA Guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees) related to the Regulation (EC) 1107/2009 valid test methods...

26 March 2020 1,178 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View