I would like to stain for Epidermal Langerhans cell in rat skin. The problem is I am not sure what primary antibody would be the most appropriate for immunohistochemistry. I currently have CD103 (OX62) AND CD11b (OX42) but they both do not specifically label Langerhans cells in rats. What other antibodies can I use?
Are you on Twitter? Here’s a link to a great picture (and mention of antibody): https://twitter.com/smlungpathguy/status/802389186833694720
Dear Christabel,
Mac2 and Mac3 antibodies work well.
Proc Natl Acad Sci U S A. 1983 Jun; 80(11): 3448–3451. PMCID: PMC394061
Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells.
K A Haines, T J Flotte, T A Springer, I Gigli, and G J Thorbecke Copyright and License information ►This article has been cited by other articles in PMC.
Abstract
Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and-3, Ia antigen, Fc fragment receptor and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationship of Langerhans cells to the interdigitating cells of lymphoid tissues.
Regards, Fred
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