Why is self plagiarism wrong?

I've heard scholars say they could reuse their words in different papers. Where should the boundaries be drawn?

08 September 2018 905 27 View

How will I do PCA and interprete analysis in PQMethods?

I'm trying to do Q sort on PQMethods for Windows. I've entered all the sorts and I opted for PCA, varimax, then Q-analysed. I then viewed the.lis file, but need further help on interpretation and...

07 August 2018 4,070 2 View

What is the most economic method of determining plasma adiponectin levels when you have up to 1000 individuals?

If kits would be used for determining the adiponectin levels, kindly provide details of how I can get the kits.

02 March 2015 854 7 View

Can saliva be used instead of blood samples for human SNPs?

08 September 2014 2,170 8 View

Setting boundaries to avoid self plagiarism

I've heard scholars say they could reuse their words in different papers. Where should the boundaries be drawn?

01 January 1970 8,787 10 View

Peer review tip for starters

Reviewing manuscripts of articles is usually not taught and most early or mid career researchers find it difficult. I ask for suggestions on this in order to allow the starters to have the...

01 January 1970 9,933 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Can primary antibodies bind to analyte coupled magnetic beads directly if the coupling was unsuccessful ?

I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...

08 November 2020 9,911 7 View

Which organic solvent should I choose to make a cortisol stock solution for immunoassay?

Hi everyone, I plan to make my own cortisol standards for immunoassay. Hydrocortisone (cortisol) is soluble in organic solvents, such as ethanol, methanol, DMF etc., but I'm not sure which one...

01 September 2020 3,442 4 View

How can I make the Gold-Antibody conjugate stable ?

I did competitive lateral flow immunoassay using gold bridge method. Antigen-fluorescence bead and immobilized antigen on the PMMA membrane is no problem to measure the optical absorption at 37...

24 August 2020 2,684 4 View