Can I use a spectrophotometer in the place of an absorbance plate reader, to measure plasma adiponectin using R&D immunoassay kits?
Yes, The enzyme activity is measured spectrophotometrically by increased absorbance at 450 nm, corrected for the absorbance at 590.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949341/
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/se120138bul.pdf
Yes, providing you carry out the two wavelength measurements which would otherwise be corrected for using the plate reader you can used a spectrophotometer with these kits.
Thanks to all
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If kits would be used for determining the adiponectin levels, kindly provide details of how I can get the kits.
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