What blocking buffers can I use for a Lateral Flow Immunoassay?

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How to convert HIV Copies/mL to IU/mL ?

I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...

08 September 2019 3,633 4 View

Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

01 February 2019 4,179 11 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

11 December 2018 4,943 10 View

Which are the suitable gene target for DNA Extraction Internal control for RT PCR of DNA Virus?

We want Internal Control for DNA RT PCR, which should be act as a DNA extraction control.

05 June 2018 3,722 4 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

03 April 2018 5,287 4 View

I am not getting Perfect Sigmoidal curve in RT PCR. Can any one suggest the Possible reasons?

Please suggest possible modifications in experiments.

10 November 2017 393 6 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

09 October 2017 4,590 3 View

What is the best way to detect MicroRNA , Sybr Green or Taqman?

Which method is best for microRNA detection.

10 November 2015 948 8 View

Problem in R for generating Heatmap.

i am getting following error in R while generating heatmap. Error in .External.graphics(C_layout, num.rows, num.cols, mat, as.integer(num.figures), : invalid graphics state Please Guide me.

01 February 2015 8,232 3 View

Problem in Real Time PCR?

I optimised RT PCR, and its working very good, but suddenly i lost getting all the results, even positive control also. Please guide me the problem.

31 December 2014 5,370 4 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

Substitutes for Dako anti-HBcAg rabbit polyclonal?

I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...

28 April 2024 3,860 1 View

How do results of Bland-Altman plots and regression analysis contribute to our understanding of the comparability between serum tubes and LIH tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...

09 April 2024 7,249 1 View

Aside from the diagnostic efficacy of the various immunoassays for HDV, do these tests vary in risks or health hazard for the medical technologist?

Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...

09 April 2024 4,044 1 View

What are the factors that make lithium heparinized plasma (LIH) tubes more viable for use in clinical laboratories?

The journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" aims to assess the viability of replacing serum with plasma samples in...

08 April 2024 3,341 3 View

Is it economically advantageous to purify polyclonal antibodies for lateral flow immunoassays?

Dear researchers, I am interested in the purification process of polyclonal antibodies for use in lateral flow immunoassays. Specifically, I would like to inquire about the common practices and...

14 February 2024 9,120 2 View

Insulin unit conversion method ?

"Using the Insulin unit conversion method (1 µIU/mL = 0.04 ng/mL), can this be applied to measurements taken by ELISA (pg/mL) rather than ECLIA (Electrochemiluminescence Immunoassay)? This...

01 January 2024 7,504 1 View

What does the meaning of numbers that given in the Result of qualitative type of ELISA?

I am working with NOVATECH ELISA KIT for Brucella. In the leaflet, it is mentioned that it is a qualitative assay with a cutoff starting at 11. When I enrolled the assay through the DS2 automatic...

09 December 2023 6,166 3 View

How can the quantity of particles accumulating at the sample pad-nitrocellulose membrane interface in a lateral-flow immunoassay be reduced?

Hello, I am performing research with the lateral-flow immunoassay (LFA) and am experiencing a large quantity of particles getting stuck at the interface between the fiberglass paper sample pad...

16 November 2023 4,785 3 View

We have Bio-Rad iMark Microplate reader in our lab. How could we use this instrument in plant biology research? What biological assays could we can?

About application of Bio-Rad iMark Microplate reader beyond immunoassays.

13 July 2023 295 3 View