Hello. I am interested to hear advices on how to measure HAT activity from immunoprecipitated samples. After preparing a cell lysate, I have been using a HAT activity assay kit with spectrophotometric analysis (detection of NADH upon reaction with a soluble tetrazolium dye, EPI001 Sigma Aldrich). In addition, does it play a role whether I use denaturing or undenaturing elutions in IP before analysis of HAT activity? I have used undenaturing conditions to preserve protein-protein interactions.
Goodwin G. Jinesh
Regarding above paper, WB for acetylated histones will help to avoid using radioactivity.
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Elina H. Mäntylä
Great! Thank you for your answers! My aim is to measure if a protein has a HAT activity.
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