I have a plate made of ABS (something like 96-well ELISA plate) with square-shaped wells. I intend to use it to do dot immunoassay. ABS is hydrophobic so buffer solutions do not spread over the wells, but accumulate in one corner of the well.
To study Wiertz and the other descriptions of methods is right thing to do. As coating is a laborious technique, most folks prefer to order coated wells and kits for ELISpot nowadays. The result is even after many years they hardly understand what they are doing in their plates...
~10-15 s in Oxygen plasma cleaner
or several minutes in UV/ozone cleaner
effect will be good for the next half hour at least, I think.
Dear colleagues. Thank you for your answers. What do you think is it suitable to apply method described by Wang et al (10.1016/j.wasman.2014.02.005)? I mean about-hour-long boiling treatment.
As Dustin said, the O2 plasma cleaner should make your surface more hydrophillic. But from my experience (with PC and COC), it won't last for more than 10-15 min....
I have the impression all such techniques are not perfect for the task. We treated plates with MgSO4 solution overnight at +4 before antigen coating, and also it is possible to treat plate with 96-percent ethanol before MgSO4. It is approximately the same like boiling or UV maybe, coating effect is 10-50 percent improved only. Different type of plastic can be more useful if it still doesn't work.
by using oxygen plasma treatment you can make your surface more hydrophillic but it's temporary.
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