cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

17 September 2017 3,410 10 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

08 September 2017 229 11 View

Does the use of RNAse inhibitor is mandatory in Reverse-Transcription experiments?

I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...

19 April 2017 927 4 View

Has anyone already used the estradiol ELISA kit for bovine samples from ABNOVA (ref. KA2276)?

28 January 2015 5,715 2 View

Has anyone already used the estradiol ELISA kit for bovine samples from ABNOVA (ref. KA2276)?

31 December 2014 4,599 2 View

Does anyone know a commercially available ELISA kit able to measure prolactin in bovine plasma samples?

Prolactin being a protein hormone, it is species-specific and I could only find prolactin ELISA kit in dogs (and humans of course).

16 June 2014 6,906 4 View

Does anyone know a commercially available ELISA kit able to measure prolactin in bovine plasma samples?

Prolactin being a protein hormone, it is species-specific and I could only find prolactin ELISA kit in dogs (and humans of course).

05 June 2014 3,791 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Can primary antibodies bind to analyte coupled magnetic beads directly if the coupling was unsuccessful ?

I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...

08 November 2020 9,911 7 View

Can someone recommend how to stain epithelial cells with carmine powder?

I need to stain mouse mammary epithelial cells with carmine to detect prolactin. I have the carmine in powder form.

22 September 2020 6,884 4 View

Which organic solvent should I choose to make a cortisol stock solution for immunoassay?

Hi everyone, I plan to make my own cortisol standards for immunoassay. Hydrocortisone (cortisol) is soluble in organic solvents, such as ethanol, methanol, DMF etc., but I'm not sure which one...

01 September 2020 3,442 4 View