I have never used it and would like to know if others have been satisfied with it, especially regarding its accuracy and precision.
We would like to assess the chitinase activity in fish mucus sample, and have started to work with a chitinase assay kit from Sigma. Unfortunately, this one is out of stock since several...
26 May 2020 8,522 3 View
Has anybody already perfomed CSF punctures in rabbits via the cisterna magna and could give me technical advice about it? How much CSF can be collected by this way?
31 May 2018 2,918 3 View
Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!
17 September 2017 3,567 10 View
08 September 2017 369 11 View
I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...
19 April 2017 1,090 4 View
Surprisingly the provider (Abnova) does not recommend an extraction of estradiol from bovine sera samples before the assay, unlike protocols from other estradiol ELISA kit, and the sensitivity...
28 January 2015 5,886 2 View
I would appreciate details regarding the extraction procedure such as the drying method (if so), the extraction buffer (methanol, ethanol...), and the assay method in EIA. Thanks.
08 January 2015 1,729 1 View
31 December 2014 4,730 2 View
We would need a device with a container large enough to collect the whole urine and tough enough to withstand the flow strength... and preferably commercially available. Thanks
01 October 2014 8,220 10 View
Prolactin being a protein hormone, it is species-specific and I could only find prolactin ELISA kit in dogs (and humans of course).
16 June 2014 7,042 4 View
We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...
25 May 2024 7,663 3 View
I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...
28 April 2024 3,860 1 View
In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...
09 April 2024 7,249 1 View
Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...
09 April 2024 4,044 1 View
The journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" aims to assess the viability of replacing serum with plasma samples in...
08 April 2024 3,341 3 View
Dear researchers, I am interested in the purification process of polyclonal antibodies for use in lateral flow immunoassays. Specifically, I would like to inquire about the common practices and...
14 February 2024 9,120 2 View
"Using the Insulin unit conversion method (1 µIU/mL = 0.04 ng/mL), can this be applied to measurements taken by ELISA (pg/mL) rather than ECLIA (Electrochemiluminescence Immunoassay)? This...
01 January 2024 7,504 1 View
I am working with NOVATECH ELISA KIT for Brucella. In the leaflet, it is mentioned that it is a qualitative assay with a cutoff starting at 11. When I enrolled the assay through the DS2 automatic...
09 December 2023 6,166 3 View
Hello, I am performing research with the lateral-flow immunoassay (LFA) and am experiencing a large quantity of particles getting stuck at the interface between the fiberglass paper sample pad...
16 November 2023 4,785 3 View
About application of Bio-Rad iMark Microplate reader beyond immunoassays.
13 July 2023 295 3 View