Can sgRNAs for CRISPR Cas9 be introduced into the cell as an oligonucleotide?

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What are the conflicts between EIA and environmental engineers and lawyers?

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Multicloning in pRMC2?

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How to convert a domestic microwave assisted synthesis for dedicated instrumentation?

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Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

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How do you improve retroviral expression from PG13 cells?

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Numerical taxonomy with SPSS?

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How to determine the wavelength of an organic molecule ?

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Adding xanthan gum to aid particle suspension during laser particle size analysis?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

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How can I completely delete a protein on the genomic level using Crispr?

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Choise of cell line for neurobiology?

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Should GFP sequence be cut together with the IRES sequence?

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Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

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31 January 2021 2,516 1 View

How to do DNA normalization based on qPCR values?

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Does anyone has a protocol for transfecting AML12 cells?

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Should I add a promoter upstream of my CRISPR-inserted gene?

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Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

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Abel Tan

Yes its possible. Heres a kit:

https://www.idtdna.com/pages/docs/default-source/catalog-product-documentation/crispr-2-part-rna-transfection.pdf?sfvrsn=11

You may want to use higher amounts of sgRNA compared to donor.

Bhavnesh Kumar

Depending on the working model system one can deliver the sgRNAs as oligos directly into the cell. Micro-injection has been successfully, or for better results synthesize sgRNA and the deliver sgRNA in RNA form using any efficient RNA transfection reagent.