Can sgRNAs for CRISPR Cas9 be introduced into the cell as an oligonucleotide?

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Multicloning in pRMC2?

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Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

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How can I completely delete a protein on the genomic level using Crispr?

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Choise of cell line for neurobiology?

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Should GFP sequence be cut together with the IRES sequence?

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Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

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How to do DNA normalization based on qPCR values?

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Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

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Abel Tan

Yes its possible. Heres a kit:

https://www.idtdna.com/pages/docs/default-source/catalog-product-documentation/crispr-2-part-rna-transfection.pdf?sfvrsn=11

You may want to use higher amounts of sgRNA compared to donor.

Kevin Farol Kanda

Thank you for your suggestion and the link to the kit. We'll definitely try it out.

Bhavnesh Kumar

Depending on the working model system one can deliver the sgRNAs as oligos directly into the cell. Micro-injection has been successfully, or for better results synthesize sgRNA and the deliver sgRNA in RNA form using any efficient RNA transfection reagent.