Hello

links & attachment here will help you

Good Luck 

https://www.jove.com/video/5064/immunohistochemistry-protocol-for-paraffin-embedded-tissue-sections

https://insitutech.wordpress.com/2016/01/28/immunohistochemistry-on-paraffin-sections/

https://link.springer.com/protocol/10.1385/1-59259-678-9%3A77

https://www.researchgate.net/post/Has_anyone_tried_an_in_situ_in_adult_zebrafish_tissues

http://www.sciencedirect.com/science/article/pii/S2468550X17300746

http://animal.ifas.ufl.edu/hansen/lab_protocol_docs/antigen_retrieval_immunohistochemistry.pdf

https://www.nature.com/nprot/journal/v5/n6/pdf/nprot.2010.62.pdf?origin=ppub

thank you

Sofia Macedo

Hi,

I use H2O2 only for endogenous peroxidase activity blockage. For antigen retrieval, I normally use sodium citrate.