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Questions related to Histological Techniques
I have paraffined goat organs for histology and would like to know if there are any difference in the presence of the enzyme gluthathione peroxidase in some organs and tissue between animals...
26 February 2016 6,987 2 View
How to calculate the dose needed for injection in this muscle in rabbit? what is the scientific basis for calculation of the dose?
20 February 2016 6,779 2 View
Immuno-Histochemistry stained cells with GFP and DAPI and want to know how to quantify the positive cells.
15 February 2016 8,472 3 View
I've stained trichodinids & sessile ciliophorans for many years using Mayer's & Harris's haematoxylin successfully. Lately a few batches of stains destained few months after initial stain....
12 February 2016 1,139 2 View
I am trying to visualize under confocal microscopy some GFP tagged proteins after I treated the cells with sodium azide during 30 minutes at 30ºC. I just take the cells at OD 0,4-0,6 in SC media...
11 February 2016 5,929 1 View
I need coronal sections of the substania nigra and VTA for confocal fluorescent imaging. When cutting in the brain on the cryostat, what are some useful landmarks I can use (looking grossly) to...
10 February 2016 6,309 3 View
I am working on mouse brain coronal section. I am doing a quantification of activated cells in several areas by cFos staining. I am having a hard time doing it on the auditory cortex. I wonder If...
04 February 2016 2,737 1 View
I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...
02 February 2016 9,202 18 View
I have tried to perform Live/dead assay on my seeded decellularised trachea scaffolds approximately 2-3mm thick,, were I have washed my biopsies initially with PBS then stain with Calcein Am/...
02 February 2016 767 4 View
Hi all scientists here in this group-help needed to find out how to count bacteria (green/red) in an image from fluorescence microscope? I have green and red bactria to count from images, for...
02 February 2016 9,164 1 View
02 February 2016 5,911 1 View
At the time of the primary antibody (FSH) added a solution of chromogen DAB, the positive control was stained in the pars nervosa but in many journal said FSH was faound at pars distalis of...
02 February 2016 5,795 3 View
I have tried to do using 1ml Tris-EDTA Buffer , pH -8.2, tissue homogenate 50 micro lit and 1 ml pyrogallol (0.2 mM). But results is not good as rate of change for homogenate is higher for some...
02 February 2016 4,638 2 View
Hello! I am looking for some suggestion about the staining on acute rodent brain slices with tomato lectin in order to visualize with 2 photon microscopy the living microglia. I am not sure about...
02 February 2016 7,095 2 View
Hey all, we're trying to get images from H&E stained kidneys using NIS Elements and a Nikon DS-FI1 camera. The images are all too dark on the camera, though through the ocular they're fine....
02 February 2016 5,946 0 View
I tried sonication and homogenizer and then pestle and mortar method as well but the protein concentration is very low bcz of which I am unable to get bands.
02 February 2016 9,505 4 View
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02 February 2016 6,270 4 View
we want to detect alpha estrogen receptor in ovary and uterus of rat with IHC method. But in the result, the negative control stil stained and it he granulosa cell was not stained.
02 February 2016 6,724 12 View
02 February 2016 6,946 1 View
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02 February 2016 7,357 2 View
Hi everyone, I'm very interested in studying mouse subventricular zone (SVZ) sections. According to literature, vibratome cutting provides the best approach possible to observe this structure. As...
02 February 2016 2,298 2 View