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Questions related to Histological Techniques
Before putting the tissue into the OCT compound, we know that it needs to be fixed and dehydrated through a pretreatment process. My method is to store fat tissue, liver tissue and skin in 4%...
13 March 2017 8,781 1 View
For immunohistochemistry tissue samples are normally mounted on slides coated with poly-l-lysine. But these paraformaldehyde fixed tissues keeps falling of newly coated slides. The thickness of...
13 March 2017 9,969 3 View
I am having issues with a histology technique. I'm currently cryosectioning sciatic and L4 spinal nerves, followed by staining with toluidine blue. My section are coming out nicely but quickly...
12 March 2017 3,304 3 View
OTC frozen brain tissue Concentration and staining time suggestions are welcome
10 March 2017 9,394 2 View
I got some brains (P1 and E.17 mouse brains) from a collaborator who already fixed them in formaldehyde in 2015, and then transferred to 70% ethanol for storage since then (almost 2 yrs). I...
08 March 2017 5,493 2 View
The tissue is intestine. The thickness is 8 micron. The fixation is 20 minutes
27 February 2017 3,232 2 View
I was wondering if you can do peroxidase tx and triton permeabilization on a sagittal chunk of rat brain prior to frozen sectioning and staining
13 February 2017 3,517 3 View
I am working on plastinated bone samples. After performing immuno-histochemistry (IHC) I found staining was okay but specimens were slightly damaged may be because of harsh deplastinizing process.
13 February 2017 6,694 3 View
Hi ! I recently, for the first time, tested an antigen retrieval protocol using a citrate buffer on my frozen slides. I obtained a clearer staining, and I'm willing to use this technique...
09 February 2017 7,315 5 View
Hi, I recently tested an antigen retrieval protocol using a citrate buffer on frozen slides. I obtained a clearer staining, and I'm willing to use this technique routinely. However, I think that...
09 February 2017 5,210 6 View
I just compared the prices of 5 ml 4% OsO4 solution of Sigma (184 EUR/5 ml) with Alfa Aesar (149 EUR/10 ml) and agarscientific (51,6...
07 February 2017 7,150 10 View
My lab has been attempting to work out a fluorescent in-situ protocol for fixed, frozen, rat brain sections. We're almost there, but the main issue is that whether the tissue remains adherent to...
02 February 2017 1,489 3 View
I am wanting to establish a protocol to do this kind of processing and am unable to find a clear protocol online. I understand that UV is required for curing, but also possibly a waterbath to...
02 February 2017 3,122 2 View
I am working on schedule of a big experiment and want to know the maximum time I can leave the sections in Formaldehyde after the sacrifice. Usually I pass the sections to the Machines of Fixation...
22 January 2017 7,038 2 View
After taking 250micrometer thick rodent brain section (by vibratome in aCSF) and incubate them with 1mg/ml of PI for 20 mins and then 1microgram of DAPI for next 40 min ( with/without fixing the...
06 January 2017 153 1 View
Has anybody experience with WGA staining in formalin-fixed and paraffin-embedded tissue sections? I've been trying to visualize the cell membrane in mouse heart sections, but so far I only managed...
05 January 2017 6,955 9 View
As part of one of my research colleagues at the Genetic department in a public hospital, we came across a patient with skeletal abnormality and other symptoms (not relevant for my question). We...
05 January 2017 538 1 View
Hi, Some weeks ago I sent some paraffin blocks and sections (mouse tissues from a very important experiment) to another lab and just today I was informed that the person who received the package...
04 January 2017 6,364 6 View
Can I cut the rat testis with scalpel for fixing in two different fixing solutions. also suggest me the best fixative for the rat tissues.
28 December 2016 8,774 5 View
I am planning on getting histology sections from a very small-sized sample. The section must show the center of the tissue, so I have to divide it into halves as accurately as possible before I...
23 December 2016 6,405 7 View
I have fed the 2-3 weeks old swiss albino mice with E. coli (by gavage) and am trying to get a count of these bacteria that has caused infection in the colon region. After oral gavage the mice...
21 December 2016 2,424 4 View
I have basic question - I cut my specimen on the microtome, then I put it on the glass slide and then would like to stain it in the staining jar. However, all the jars are in the vertical position...
19 December 2016 9,943 4 View
I prepared the histological tissue according to the recipe. There were no problems during cutting, but after the drying by 37°C I recognized that the tissue does not adhere to the slides. I can...
08 December 2016 3,580 3 View
I want to culture adult mouse cerebellum, but all protocols use neonatal or embrionic cells. Another question is if it´s reasoneable incubate whole cerebellum instead of slices?
07 December 2016 2,885 2 View