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Questions related to Histological Techniques
I am trying to verify if my E. coli nissle that is expressing GFP or iRFP is localized on the interior of some Multicellular Tumor Spheroids made up of the CT26 cell line. My goal is to place my...
20 March 2017 2,768 1 View
Dear colleagues, I have problem with Fast Green FCF, I used this dye to stain cotyledons. I try 0,5% Fast Green in alcohol and methyl salicylate (1:1), and 0,1% Fast Green in 96% alkohol and...
15 March 2017 1,586 1 View
I would like to cut the adipose tissue using a frozen section (10 μm) and do an oil red o staining. I have never done this before so I do not know how. Everyone seems to have a different way.The...
13 March 2017 6,940 4 View
Before putting the tissue into the OCT compound, we know that it needs to be fixed and dehydrated through a pretreatment process. My method is to store fat tissue, liver tissue and skin in 4%...
13 March 2017 8,833 1 View
For immunohistochemistry tissue samples are normally mounted on slides coated with poly-l-lysine. But these paraformaldehyde fixed tissues keeps falling of newly coated slides. The thickness of...
13 March 2017 10,033 3 View
I am having issues with a histology technique. I'm currently cryosectioning sciatic and L4 spinal nerves, followed by staining with toluidine blue. My section are coming out nicely but quickly...
12 March 2017 3,400 3 View
OTC frozen brain tissue Concentration and staining time suggestions are welcome
10 March 2017 9,462 2 View
I got some brains (P1 and E.17 mouse brains) from a collaborator who already fixed them in formaldehyde in 2015, and then transferred to 70% ethanol for storage since then (almost 2 yrs). I...
08 March 2017 5,568 2 View
The tissue is intestine. The thickness is 8 micron. The fixation is 20 minutes
27 February 2017 3,282 2 View
I was wondering if you can do peroxidase tx and triton permeabilization on a sagittal chunk of rat brain prior to frozen sectioning and staining
13 February 2017 3,607 3 View
I am working on plastinated bone samples. After performing immuno-histochemistry (IHC) I found staining was okay but specimens were slightly damaged may be because of harsh deplastinizing process.
13 February 2017 6,769 3 View
Hi ! I recently, for the first time, tested an antigen retrieval protocol using a citrate buffer on my frozen slides. I obtained a clearer staining, and I'm willing to use this technique...
09 February 2017 7,386 5 View
Hi, I recently tested an antigen retrieval protocol using a citrate buffer on frozen slides. I obtained a clearer staining, and I'm willing to use this technique routinely. However, I think that...
09 February 2017 5,270 6 View
I just compared the prices of 5 ml 4% OsO4 solution of Sigma (184 EUR/5 ml) with Alfa Aesar (149 EUR/10 ml) and agarscientific (51,6...
07 February 2017 7,235 10 View
My lab has been attempting to work out a fluorescent in-situ protocol for fixed, frozen, rat brain sections. We're almost there, but the main issue is that whether the tissue remains adherent to...
02 February 2017 1,541 3 View
I am wanting to establish a protocol to do this kind of processing and am unable to find a clear protocol online. I understand that UV is required for curing, but also possibly a waterbath to...
02 February 2017 3,191 2 View
I am working on schedule of a big experiment and want to know the maximum time I can leave the sections in Formaldehyde after the sacrifice. Usually I pass the sections to the Machines of Fixation...
22 January 2017 7,123 2 View
After taking 250micrometer thick rodent brain section (by vibratome in aCSF) and incubate them with 1mg/ml of PI for 20 mins and then 1microgram of DAPI for next 40 min ( with/without fixing the...
06 January 2017 210 1 View
Has anybody experience with WGA staining in formalin-fixed and paraffin-embedded tissue sections? I've been trying to visualize the cell membrane in mouse heart sections, but so far I only managed...
05 January 2017 7,015 9 View
As part of one of my research colleagues at the Genetic department in a public hospital, we came across a patient with skeletal abnormality and other symptoms (not relevant for my question). We...
05 January 2017 592 1 View
Hi, Some weeks ago I sent some paraffin blocks and sections (mouse tissues from a very important experiment) to another lab and just today I was informed that the person who received the package...
04 January 2017 6,478 6 View
Can I cut the rat testis with scalpel for fixing in two different fixing solutions. also suggest me the best fixative for the rat tissues.
28 December 2016 8,853 5 View
I am planning on getting histology sections from a very small-sized sample. The section must show the center of the tissue, so I have to divide it into halves as accurately as possible before I...
23 December 2016 6,464 7 View
I have fed the 2-3 weeks old swiss albino mice with E. coli (by gavage) and am trying to get a count of these bacteria that has caused infection in the colon region. After oral gavage the mice...
21 December 2016 2,485 4 View