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Questions related to Histological Techniques
Having a hard time getting a decent slide out of rat spinal cord sections ive mounted in paraffin. There's tiny streaks/tears in the section it self but not the surrounding wax. Here's the...
06 June 2016 7,353 2 View
For my stainings of 3 um thick slides of mouse liver tissue (fixed for 24 hrs at 4C, 4% paraformaldehyde) I accidentally didn´t use Triton X-100 to permeabilize cell membranes. I did it the same...
06 June 2016 6,989 10 View
I'm interested in looking at neutrophils in paraffin-embedded tissue samples and was wondering if there's anyway to determine if the neutrophils have been activated or not? And what are the best...
06 June 2016 1,539 2 View
What is the function of sucrose in lysis buffers for isolation of intact nuclei? I observed that nuclei pellets resuspend easier in buffers without sucrose, and have a higher tendency to clump...
06 June 2016 4,109 10 View
Hi all,I have two questions: 1) I need to fix the potato tissues without disrupting the cell walls and using non-organic solvents, anyone has any experiences on plant tissue fixative? 2) what...
06 June 2016 10,072 3 View
Along the lines of this question (see link) the tissue is not paraffin embedded, but will simply be kept in the formalin fixation solution Is this a viable approach for several weeks/months?
05 May 2016 1,283 3 View
I have purchased one and tried it in 4% PFA fixed and cryosection slides from fetal murine intestine. I did not get positive signal. I am going to try paraffin sections from adult intestine later....
05 May 2016 5,802 5 View
I have two different tissue sample with labelled pyramidal cells. It looks they have different signal intensity. I would like to quantify them. What is the best way to do it?
05 May 2016 2,151 4 View
I’m having troubles with dapi and phalloidin staining of bone sections. Does anybody have experience with fluorescent staining on paraffin section of mineralized bone?
05 May 2016 5,949 3 View
hello everyone,i want to know the process of cresyl violet staining in spinal cord sample of EAE mice and how much time required for post fixation by paraformaldehyde for sample?
05 May 2016 4,720 0 View
I am using rats ovarian tissue in processing Comet assay and I follow the COMPAC-50 high throughput comet electrophoresis system protocol by Cleaver Scientific Ltd.DNA dye that used was Vista...
05 May 2016 2,388 8 View
I put the tissue in A+B for 7 days and in C for 5 days.
12 April 2016 3,178 1 View
I need histochemical techniques of carbonique anhydrase révélation for 10 % formalin fixed specimens
11 April 2016 2,446 6 View
I have a peculiar microscope setup which requires me to mount cells on the thin edge of a microscope slide, has this ever been achieved? So far I've attempted using polylysine to adhere cells but...
10 April 2016 8,008 3 View
I used formalin-fixed paraffin-embedded liver tissue to do the immuofluorescent staining, but the result doesn't look good, can anyone tell me what is possibly wrong with it? I used 20% normal...
07 April 2016 1,078 9 View
Hi, I am enquiring if anyone knows any microscope platform that allows for live-display of normalised fluorescence intensity of field of view? For example if a GFP reporter that can be normalised...
04 April 2016 6,714 5 View
The experiment involves uptake of a fluorescent compound and confocal imaging. Attempts to fix the cells with 4% PFA and staining 1:50 with Alexa Fluor 633-conjugated phalloidin in accordance with...
04 April 2016 1,246 4 View
Need to do immunofluorescent staining on human tissue.
04 April 2016 5,633 3 View
I want to do the primary pinealocytes culture from the fish pineal tissue. So I need the protocol for this culture, anyone kindly suggest me any good protocol or any research article related to...
04 April 2016 5,091 4 View
more question for you to answer: Shelf life of fine fix solution. Dilution ratio of fixative with diluent? How many cassettes are processed / L. Shelf life of blocks/archival for molecular...
04 April 2016 8,296 0 View
04 April 2016 735 1 View
04 April 2016 6,222 7 View
04 April 2016 9,028 3 View
For a human biomontoring study, I am planning to collect blood samples in EDTA tubes, store at -20°c for 5 days, and then transport in ice box to the lab where the samples will be deeply frozen...
04 April 2016 2,694 2 View