Which large sustainable energy demonstration projects are running right now, which have not been reported in a scientific publication yet?

I would like to know which large sustainable energy demonstration projects are running right now, in a country or series of countries, which are too premature to already being subject of a...

03 May 2020 4,174 9 View

R 3D plot with centered axis ?

I want to represent a series of results computed from y = f(x1, x2, x3), with x1, x2 and x3 being in the [-2, +2] range. For now, I use the "scatter3d" package, and an example of a resulting...

21 December 2019 4,986 4 View

How to model proper contact definitions between bodies in ANSYS for rectangular 'pressure vessel'?

Dear all, For a project I am currently working on, the intention is that I calculate the maximum allowable pressure in a rectangular 'pressure vessel'. Of course this shape is not suitable for a...

08 January 2019 7,574 2 View

How do fungicides defeat rust disease?

Also, I would like to ask: 1. How effective are the fungicides? 2. Can rust disease develop resistance? 3. Are there already natural fungicides for rust disease? If you could provide sources to...

16 March 2018 2,135 12 View

What is the effect of dormancy on TTC viability test of embryos?

Will a dormant but living plant embryo always stain red if subjected to TTC? Or can an embryo be so dormant that it will not show dehydrogenese activity?

05 March 2018 7,673 11 View

Does anybody have experience with CATS-small-RNA seq kit from Diagenode?

I am sequencing extracellular vesicle RNA on illumina Nextgen 550, on paper for this kit looks great... but has anybody experience with it?

03 December 2016 2,405 0 View

Does Propidium Iodide have the same fluorescence yield when bound to RNA or DNA?

14 June 2016 8,094 3 View

Troubleshooting Overnight Rest of PBMCs in Serum free Media?

I am working on a protocol to stimulate PBMCs with IFNy to measure transcription activation of genes along the kynurenine pathway using rt-PCR. I want to use serum free media to minimize...

13 June 2016 1,592 3 View

How can I perform mouse cytokine ELISA on high lipid plasma?

Hi All, I'm need to perform an IFNalpha ELISA on mouse plasma samples, however these samples are from mice that received a high cholesterol diet and therefore the plasma is turbid because of the...

11 September 2014 7,970 3 View

Does anyone have experience with global analysis of gene expression, and which method is the best?

I am planning to perform a global analysis of gene expression for the first time. I will be focusing on angiogenic factors. We are using mouse samples. Does anyone have any experience with such...

05 June 2014 5,633 4 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Which software is the best to model a non-linear concrete (ABAQUS, ANSYS,ADINA, ATENA, etc.)?

What is the best finite element modeling software packages for modeling non-linear and fracture analysis of CONCRETE? I've had some with ABAQUS standart/explicit, but I would like to learn if...

27 January 2021 330 6 View