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Questions related to Histological Techniques
Hi ,I am trying to work on pepsin for antigen retrieval for formalin fixed paraffin embedded lung tissues.It did not work when I dissolved the lyophilized powder in 10mM HCl at ph 2.I want to know...
12 September 2016 1,419 1 View
I´m using the Reliaprep gDNA tissue Miniprep System from Promega. I want to know if I should wash the sample or leave it in a buffer or distilled water to wash out the ethanol before using the kit.
12 September 2016 3,038 1 View
I would like to know a reliable method of staining plant neucleolus in lathyrus sativus. I have got some old literature with allium cepa nucelolus staining. Can anyone give me the silver nitrate...
10 September 2016 6,280 2 View
Dear colleagues, I have a human aorta frozen at -80°C and I want to fragment the sample in small pieces without thawing it. Afterwards, I want to perform PCR and immunostaining. How can I do it?...
09 September 2016 3,284 2 View
Hi all! Can a mouse whole vertebral column be stored in 4% PFA for longer than 24 hours, but shorter than one week? I plan to put the vertebral columns of my mice in PFA on a Friday and do sucrose...
08 September 2016 8,329 9 View
Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...
07 September 2016 8,700 2 View
My research involve giving arsenic orally to Spraque Dawley rat. I noticed that perfusion is the standard method to isolate liver. However, since cost is my main problem to buy percoll and...
24 August 2016 7,488 3 View
I want to do p75NTR immunostaining in stressed vs unstressed brain slices in rats. What is a best antibody for this?
23 August 2016 4,442 10 View
Can any one tell me what is the best technique that can detect the concentration of thymoquinone In biological tissues ?
23 August 2016 6,880 5 View
I have tried scraping the soft tissues off using a scalpel but the whole procedure was a lengthy one. So I was wondering if anyone could tell me any other quicker way of removing the soft tissues...
23 August 2016 3,748 15 View
I'm attempting to label fixed tissue with EdU using azide 488 and another antibody using streptavidin 568. When done separate both label very well, however, when I label a tissue with both the...
15 August 2016 3,716 6 View
I am working on immunohistochemistry in testis tissue. After fixation in Bouin's fixative how long we can store testis tissue in 70% alcohol, before processing for paraffin embedding.
14 August 2016 4,673 3 View
I am performing histology on rat knee joints and would like to look at the changes in cartilage, I have fixed the joints in 10% NBF for 3 days and decalcified for 11 days in 10% formic acid. What...
12 August 2016 9,914 2 View
My goal is to establish diagnosis of NAFLD/NASH and follow-up disease progression. I obtained some H&E sections of needle biopsy from patients and mouse liver tissues. Would H&E sections...
09 August 2016 636 3 View
Having different digital images of various histological methods, especially immunohistochemistry, I want to quantify the results in terms of area, number of cells marked, and whatever else is...
05 August 2016 386 6 View
Nowadays I have experiment to analyze the skin tissue. so we have tried to make paraffin block. tissue size is about 18 x 14 x 9 mm. but we failed to section the paraffin block, just section the...
01 August 2016 5,651 3 View
Dear all, I have tried to use 40:80% Percoll to isolate tumor-infiltrating lymphocytes from tumor tissue of CT26 BALB/c mice model. But when I used this method after collagenase digestion and...
01 August 2016 5,823 9 View
I am collecting embryos for histological analysis and need to genotype them beforehand. In the past I have worked with older mouse embryos (E8.5 and up), where it is easy to remove the yolk sac...
22 July 2016 8,908 3 View
Planning on doing transcardial perfusions to clear/fix brain tissue in postnatal day 0-1 rat pups. I have never perfused anything that young/small, so I am looking for tips. Are there any special...
20 July 2016 2,601 3 View
My lab does histology of brain tissue infrequently, but in large groups. Our last batch was approximately six months ago, and we had about 100 subbed slides (chrom alum) left over after the...
19 July 2016 9,718 4 View
I am conducting four separate analyses (RNA and protein quantification, DNA methylation, and folate assay) using mice tissue, particularly the small intestine and liver. I was wondering what a...
19 July 2016 4,770 2 View
I would like to know whether it is possible to rescue tissue from paraffin embedding, so that it could be dissected and used for RNA extraction. If so, could somebody recommend a protocol for...
15 July 2016 8,595 4 View
We have only used frozen sections for autoradiograpy with PET tracers so far. One of our collaborators is interested in testing some paraffin embedded sections. Can they be used? Has someone...
13 July 2016 3,708 8 View
I'm looking for a durable matrix slicer to make coronal slicers of an adult rat heart in increments of 1 mm. I'm looking currently at Zivic Instruments and Cell Point Scientific, but am not sure...
12 July 2016 2,390 4 View