Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

How to solve 1-D Wave equation?

Good day, In standard PDE literature, there are a lot of solution approaches for the 1-D wave equation initial-boundary value problem. One way is with separation of variables. Below I describe...

25 January 2021 4,876 3 View

Journal Quartiles for Research Evaluation?

Dear all, Could you please tell me the countries where the journal quartiles SJR/JCR are used for the state system for research evaluation (except for Russia)? Best, Dmitry

21 June 2020 5,635 7 View

Could you please suggest an XRD data viewer?

Hi. I'd appreciate if someone could suggest a powder XRD (NPD, PDF as an option) data viewer which: (1) Is free of charge (2) Is easy to operate, i.e. can be run with a double-click, multiple...

06 June 2020 6,493 2 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How we can painting with powder by electrostatic method without Faraday effect?

Good day, dear colleagues I have some question for You I'm working on the powder painting of details with difficult geometry. The tribostatic method has a good result about this I try to use...

25 February 2020 2,190 3 View

Is there any sources for 661-W cell line?

Hello! For my current project I need cell line 661-W - this is a mouse ganglion precursor-like cells. I had checked many supplies (ATCC, Sigma Aldrich etc) - but none offers this line. I even...

03 February 2020 1,011 2 View

Manuscripts for the 4th volume of the "Biodiversity, biogeography and nature conservation in Wallacea and New Guinea"?

26 November 2019 1,452 4 View

Where to go to the conference?

20 October 2019 6,938 3 View

How to get correct information on the current level of development of stem cell medicine?

Stem cell research is a long-standing subject. Quite a lot is known for stem cells. But their massive use in medicine is a novelty of recent times. This area of medicine is developing rapidly....

14 January 2019 9,853 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View