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Questions related to Histological Techniques
Dear All, I wish to perform WISH on an adult zebrafish sample which is treated with collagenase and later acid-fixed (Formaldehyde + Acetic Acid). But before the WISH procedure, I would like to...
14 June 2017 6,119 1 View
I am hoping to perform in either fresh frozen or fixed mouse brain tissue. Thank you!
09 June 2017 2,923 2 View
I have the lungs, spleen and lungs of=f the BALB/c mice with me and I want to proceed for H&E staining of these tissues. But I would like to know whether any specific section from these...
29 May 2017 7,453 4 View
I wash rat heart (left ventricle) 2 times with PBS over an ice bath, dry it with paper towels and then embed it in OCT over cooling bath. Lately, while sectioning in cryostat tissue is falling...
24 May 2017 1,278 6 View
I recently performed H&E staining on my paraffin embedded P0 mice tissues to visualize the phenotype of the eye in one of my WT mice. I managed to slice the tissues and stained them with...
22 May 2017 3,106 3 View
I'm working with a gene that thins insect wings. I need a method to measure that thickness is reduced. The wings of my study organism (large milkweed bug) are extremely small and have a cuticle...
21 May 2017 3,226 2 View
Guys, which is the best protocol according to Your experience to cut ultrathin sections of human embedded spermatozoa?
17 May 2017 620 2 View
Does anybody have experience/good advice on using the DAPI stain on fresh tissue (thickness between 0.5 and 1mm)? Concentrations, preparations, incubation time? Thanks so much!
16 May 2017 4,916 2 View
We dont have enough time to embed them. The tissue is intestinal.
11 May 2017 6,704 4 View
Hello everyone, I'm a lab technician and I have a recurrent problem in cell culture : since a few months I have a viscous pellet when passaging the cells. I only have NIH 3T3 Src, a murine embryo...
10 May 2017 4,125 3 View
I need Program can make qualification to Immunohistochemistry images
05 May 2017 5,897 5 View
HI. I want to do Oil Red O staining on mice liver tissue. I have either parafin embedded tissue or frozen tissues that i have frozen in liquid nitrigen after cutting from mice and then at -80. Can...
04 May 2017 8,878 3 View
I am having problems fixing the mucus layer on both mouse colon sections and human colon cell line (which secretes mucus) grown on transwell. I am using methanolic carnoy's fixative, which is...
04 May 2017 2,561 1 View
Everyone ever do the immunohistochemistry without tissue being fixed with formaldehyde? After sacrifice the animal I directly put the tissue into the 30% sucrose, for further cryosection, I...
04 May 2017 4,694 6 View
I am doing immunohistochemistry (IHC) in Prefrontal cortex of brain section slices after fixing by formalin 4%. My IHC images have so many white vacuole, has anyone who has experienced this...
01 May 2017 10,094 7 View
I am interested in looking at skeletal muscle histology, H&E staining, and plan to fix tissue in 10% NBF, but can't process tissue until later. What solution can I use to store this tissue?...
26 April 2017 6,280 7 View
I'm planning to store some histology slides that I've made with a cryostat, normally I keep them frozen, but is there a way I can keep them long term without degradation at room temp? As that...
24 April 2017 4,111 0 View
Hello everyone I recently started working on a project concerning the RPE and the retina. I want to perform IHC on paraffin embedded eyes, but I have problems to find the right fixative because...
19 April 2017 9,594 5 View
Hi, I will soon be preparing frozen mouse lung tissue for immunohistochemistry. I hear that with formalin based fixation, immunohistochemistry would be difficult to perform. I have also read...
16 April 2017 4,058 8 View
Hi Guys, I am working on western blotting of tight junction proteins in mice colon tissue. Currently, I suspect my problem is low protein content in the prepared lysate. I am just wondering...
15 April 2017 490 1 View
I want to do rat brain immunohistochemistry, and I find that rats were perfused transcardially with PFA under deep anesthesia in all published papers. But now some people in pathology told me that...
12 April 2017 7,956 5 View
I tried using egg albumin but there were non-specific binding all over the sample with my probes. I read on using gelatin with chrome but it also had the same comments on probe non-specific...
28 March 2017 9,042 1 View
Hi, I have been doing slide in situ hybridisation on wax sections (mouse embryos age e10.5) and every time I do it the tissue sections look damaged. Can anyone suggest a solution to improve the...
24 March 2017 1,704 2 View
Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and...
23 March 2017 6,570 4 View