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Questions related to Histological Techniques
I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....
15 March 2016 1,656 8 View
Hi everybody When I get fresh frozen tissue (without fixating with PFA or formaldehyde, just snap frozen with OCT) sliced with the cryostat mounted in a slide it doesn't immediately get attached...
11 March 2016 8,108 1 View
I am staining mouse skin cryosections (10um) with PanH3, an antibody that binds to histone 3. When harvesting this tissue I embed in OCT and freeze using dry ice and liquid nitrogen. When viewing...
10 March 2016 7,125 5 View
I am presently using 10% formalin for fixation of the micro-dissected tissues. They are 300µm in height and a diameter of approximately 420µm. We are presently trying various test at different...
08 March 2016 4,203 5 View
I am using a plastic wrap to pick up the section and transferring to a glass slide. Unfortunately, when I take off the plastic wrap some of the section attached to the plastic wrap. Thanks for...
08 March 2016 6,563 9 View
First question here so bear with me if something is unclear: Would anyone have an idea as to wether it is possible to extract RBCs (from fish) that has a) dried up in an EDTA vacutainer for...
04 March 2016 6,512 3 View
I would like to dissociate epithelial cells from zebrafish tails and then do FACs and eventually RNA Seq. Does anyone have a good protocol that will give me good dissociation without destroying...
03 March 2016 9,095 2 View
Hello, I have an Annexin V-FITC antibody that I use to detect apoptosis on the FACS. I was wondering if I could use the same antibody to stain for apoptosis on histology slides? Thankyou!
03 March 2016 8,345 5 View
Is there anybody tried preparing intestinal tissue from rodent for identification of Cryptosporidium? please advise.
03 March 2016 3,743 3 View
03 March 2016 4,231 4 View
I need information regarding testicular fixation procedure particularly for fixed testicular of cow and goat. I have tried fixation using paraformaldehyde 4% and neutral buffer formalin but the...
03 March 2016 6,176 18 View
03 March 2016 8,619 9 View
Does anyone have a protocol for CIP treatment of paraffin sections to confirm phosho-specificity of an antibody in immunohistochemistry? I can't find details online like how much CIP, buffer, and...
03 March 2016 9,882 3 View
Samples were formalin fixed or 4% PFA fixed. Decalified in EDTA 14% for 2-3 weeks. I see that the TRAP has been used by some. But does anyone know if the ALP works too? Or any protocol to see ALP...
03 March 2016 4,019 2 View
Hi, I am looking for good and simple positive controls / marker substances to inject into ex vivo normal human skin tissue samples that give very strong and unique color signals in subsequent...
03 March 2016 5,505 3 View
what is the maximum time limit for doing Immuno Histochemistry in paraffin embedded tissues? will the antigens survive in the tissue for long time?
03 March 2016 6,090 4 View
Dear All: Does anyone know how to distinguish nervous tissue from endocrine tissue histochemically? Thanks
03 March 2016 3,180 4 View
We already used ChAT antibody to immunostain the lower motor neurons in the spinal cord and we could see the ChAT positive cells. But now we want to see the upper motor neurons in the motor cortex...
03 March 2016 5,595 2 View
03 March 2016 1,037 6 View
I am going to try detecting GFP and tdTomato expressed by Candida albicans in the murine GI tract. The previous antibodies I tried cross-reacted with Candida albicans proteins by Western blot so...
03 March 2016 9,538 1 View
03 March 2016 9,593 5 View
I would like to know if i have to finish the fixing process immediately or can wait for a while.
03 March 2016 7,978 11 View
03 March 2016 6,490 3 View
Hello! I am looking for some suggestion about the staining on acute rodent brain slices with tomato lectin in order to visualize with 2 photon microscopy the living microglia. I am not sure about...
29 February 2016 3,938 2 View