RIPA is the best way to get the membrane bound proteins right?
But has anyone tested Trizol isolated proteins to see if we get membrane proteins?
No, Trizol is not the best method for isolating membrane bound proteins. Trizol is a reagent containing phenol and guanidine thiocyanate, and is designed for isolating RNA. While it can also extract DNA and proteins along with RNA, it is not optimal for membrane proteins.
Hello Lohit,
Yes, RIPA is the best for membrane bound proteins. I have not tried TRIzol for membrane bound proteins, but there are studies that have used TRIzol to isolate proteins. The drawback of using TRIzol is that protein retrieval is difficult due to inefficient solubilization of precipitated protein pellet.
However, the papers provided below have come up with simple modification to the TRIzol protocol that help to improve protein recovery. They also claim that this protocol preserves phosphorylation of the isolated proteins. Also, cytosolic and nuclear proteins can be easily extracted and detected by immunoblotting. There is no mention whatsoever about membrane bound protein isolation by TRIzol method. l somewhat feel that TRIzol method may not work for membrane bound proteins.
References:
https://doi.org/10.1186/1471-2164-14-181
https://doi.org/10.2144/btn-2020-0083
Best.
No , Trizol using for the extraction of RNA , DNA and protiens from tissues or cell , but it is not preferable to use isolating membrane bound proteins
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