Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

How To Find Electrical Nanodipole and Magnetic Loop Dimensions At Optical Wavelength?

I would be grateful if you could suggest a paper or book which provides an equation to determine the dimensions (length) of an electrical nanodipole and magnetic loop antenna at desired optical...

17 October 2020 2,421 4 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Could anyone help me searching for protocols in fiber analyses ?

Hello, I am looking for protcoles to evaluate the following components: TDF : total dietary fiber, SDF : soluble dietary fiber, IDF : insoluble dietary fiber et TDN : total digestive nutrients.

06 September 2020 371 2 View

Which Digital Elevation Model is better for Non point source pollution modelling?

Out of freely accessible Digital Elevation Model Which one is better suitable for Non-point source pollution modeling? (Indian terrain) I want to apply hydrological modeling for the study. The...

04 August 2020 8,204 3 View

In my study there are 1 independent, 5moderator and 3dependant, and only 1DV is correlated, should I perform regression and moderation for all ?

Only one dependent is correlated with independent, now regression and moderation analysis should run for all variable or only one variable which is corelated?

29 July 2020 4,470 6 View

Any journal which has no publication fess for pops removal or water treatment through adsorption?

I am looking for a reputable journal for publication of research article of MS thesis. Kindly suggest me some journals related to water treatment through adsorption which is free of cost.

13 July 2020 8,259 6 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

How to overcome problems with introduction of modified DNA into C. glutamicum?

09 December 2020 7,715 5 View

Thin cut brain tissue flaking off of slides?

Hello! I have just encountered a new issue with some tissue flaking off of my slides and wondered if anyone else has experienced this or has any suggestions? The sections were cut at 18 microns...

09 December 2020 4,954 2 View