In alpha amylase inhibitory activity, while taking UV and, do we zero the absorbance of blank or control?

02 October 2023 1 4K Report

I have to conduct alpha amylase inhibitory activity but I m confused while taking readings from uv-vis spectrophotometer regarding blank and control. Do we have to zero the blank and then take readings for control and sample?

Paul Milham

In general one zeros the instrument using a control such as pure water and measures the 'blank'. However, there are exceptions where the large size of the zero standard may cause a measurement problem, e.g., due to the performance limitations of the spectrophotometer.

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