Hi. I am currently studying umbilical cord-derived mesenchymal stem cells (UC-MSCs) and have recently induced both adipogenic and osteogenic differentiation in our experiments.
For adipogenic differentiation, we used 10% FBS along with indomethacin, dexamethasone, and insulin. For osteogenic differentiation, the medium included dexamethasone, ascorbic acid, and β-glycerophosphate. It has now been approximately 18 days since the induction.
In the adipogenic group, we observed clusters of white, translucent droplets in the cytoplasm under a light microscope, which resemble grape-like structures—presumably lipid droplets. However, in the osteogenic group, we have not observed any noticeable morphological changes so far.
Some researchers have mentioned that calcium deposition can be observed during osteogenic differentiation. Is it possible to visualize this calcium deposition prior to staining (e.g., with Alizarin Red S)?
Yes for lipid dropplets. However lipid dropplets in UC MSCs are very small compared to
For osteogenic differentiation, sometimes you can see like there is dirt on the surface of the cells, but more often nothing can be seen, though alizarin staining can be positive
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