I am trying to establish an inflammatory injury model in HK-2 cells using LPS stimulation, but I've encountered some issues.
I treated the HK-2 cells with a range of LPS concentrations (0, 1, 2, 5, 10, 20, and 50 µg/ml) in 1% FBS medium for 24 hours. However, the CCK-8 assay showed no significant differences in cell viability across the groups.
Has anyone encountered a similar situation, or does anyone have suggestions on optimizing the model? Could it be related to serum concentration, LPS batch, or cell sensitivity?
Rongzong Qiu ,
HK-2 is a human renal proximal tubule epithelial cell line. It has been confirmed in the literature (PMID: 36523420) that after 24 h of intervention with 20.0 μg/mL LPS, HK-2 cell viability decreased (CCK-8) and apoptosis rate increased (FCM).
The literature used RPMI-1640 to culture HK-2 cells, and then used for LPS intervention and activity verification. You may refer to the literature to adjust the reagents used. While use CCK-8 reagent (10 μL) for 2 h.
We (MedChemExpress, MCE) provide high-quality RPMI-1640 culture reagent (HY-K3004). Please contact us for further needs or experimental consultations.
The answer to this question comes from MCE Technical Support.
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