A forum to address questions regarding enzyme-linked immunosorbent assay, most commonly known as ELISA. | Contact experts in ELISA to get answers
1,306 views 2,618 posts
Questions related to ELISA
I have one day old chick with mycoplasma gallisepticum and Synovi ELISA titters are 1,743 and cv 68 / 3,608 and cv 30 respectively Are these titters indicates infection or maternals antibodies...
04 April 2019 8,005 4 View
Hi there. Is there anyone who is using FlexMap 3D system for multi-analyte ELISA? For multiplex cytokine measurement at one time, Luminex technology has been used. The most well-known machine...
04 April 2019 4,072 2 View
We are planning to analyse 8-isoprostane from plasma and urine samples (ELISA, Cayman). According to the manufacturers instruction we will store the samples on BHT (Store -80, seems to be...
04 April 2019 7,513 3 View
I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...
04 April 2019 9,038 13 View
I calculated LOQ and LOD for ELISA but it seems that the LOQ smaller than LOD. According to EPA (1996) and Armbruster & Pry (2008), LOQ cannot be lower than LOD. Where did I could possibly go...
04 April 2019 8,233 4 View
Hi, I am developing a sandwich ELISA for Rubella viral quantitation. I'm using Rubella Glycoprotein E1 for coating and a polyclonal HRP conjugated Ab for detection. In my last experiments, I...
04 April 2019 5,379 3 View
I am using the ELISA kits from R&D Systems and am supposed to let the plates incubate with color buffers for 20 minutes before reading. I accidentally pulled the plates out at 15 or 17 minutes,...
04 April 2019 9,106 5 View
I am looking to perform a whole cell ELISA with pertussis.
04 April 2019 485 1 View
I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...
04 April 2019 9,740 4 View
I am looking for sandwich ELISA kit specifically for the quantification of alpha7 nicotinic acetylcholine receptor proteins (CHRNA7) in rat brain tissue homogenates. I've found only these...
04 April 2019 887 3 View
Hello, I have a curious situation. I have been biopanning against antigen. My phagemid vector has an amber stop codon which allows for expression of soluble antibody into bacterial supernatant...
04 April 2019 3,551 6 View
Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have...
04 April 2019 1,519 5 View
Good morning all, My question is just like in title. I was using EDTA when collecting mouse blood, and most of ELISA kits are dedicated for plasma gained from blood collected with citrate or...
04 April 2019 6,365 2 View
I immunized mice and bovine against an specific Ag: Ag_X. I separately tested each animal serum (1 in 250) against the Ag_X, and I got good results using negative controls. I only got enough...
04 April 2019 5,018 10 View
I'd like to test the enzymatic function of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) in cell culture after knocking down the PFKFB gene using shRNA. However I have yet to find...
04 April 2019 6,527 6 View
Just to notify that at this stage I'm using the follow formula: -> Cut-off = mean + (3 x standart deviation) But I have my doubts if it is the best option. Thanks in advance. Adéritow Gonçalves.
04 April 2019 7,707 2 View
Hi! I am searching for ELISA Polycarbonate ( the wells should entirely be made of polycarbonaTe) microtitleplates. does anyone know a seller ? thanks
04 April 2019 9,193 2 View
I am trying to bind lipid extractions from mycobacteria to Nunc PolySorp 96 well plates to perform ELISA's. Lipids are insoluble in water, so when I try and dilute down my lipid fractions (in 2:1...
04 April 2019 6,566 3 View
currently we are working on various analgesics and anti-inflmmatory drugs for mechanistic evaluation. In this regard we are looking for procedure to evaluate PGE2 from paw (carragenan method),...
04 April 2019 1,422 3 View
I'm to test LTB4 on human serum samples using Cayman Chemical kit cat#520111. Has anyone done this and if so did you need to purify the samples ?
04 April 2019 6,929 1 View
I am growing adherent primary epithelial cells in supplemented DMEM/F12 and testing the supernatant for inflammatory cytokines. I purchased a commercial sandwich ELISA to test for analyte....
03 April 2019 1,807 3 View
We research a colorimetric method for assaying lipoprotein lipase activity in the supernatant by using an ELISA reader.
26 March 2019 3,289 3 View
My students are culturing mixed mouse splenocytes in culture for 48 hours with anti-CD3/28. We are then going to analyse for the presence of Tregs and measure IL10 secretion by ELISA. In order to...
21 March 2019 6,018 3 View
I want to measure hormones ( AMH, LH, FSH, estradiol) in blood samples from rat. Which one of the blood collection techniques are better for this kind of annalysis? Assays I am using don't specify...
18 March 2019 3,925 6 View