Any reccomendations for an ELISA microplate washer?

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Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to perform a mixed-effects Poisson regression with mutliple DVs?

I have a set of 6 related likert-type DVs, a within-subjects time variable (i.e. each DV was measured at 3 time points), and a categorical between-subjects IV (condition). Is there a way to...

16 December 2020 9,798 10 View

Repeated measures ANOVA with technical replicates?

I am doing an experiment where I measure the blood pressure of a treatment and control group over the course of several weeks. At each measurement session I collect multiple recordings. From what...

06 December 2020 531 7 View

Top-coded age and regression?

What is the best way to deal with top-coded age while doing regression analysis. If you are examining the presence or absence of a disease as your outcome, and a number of independent variables...

13 October 2020 907 5 View

Is there a guide that will help interpret Ponceau S staining for western blots?

Greetings, I'm having difficulty understanding my results from doing a ponceau S stain on a nitrocellulose membrane after western blot. I loaded 10ug in lanes 2,4, and 6. 15ug in lanes 3,5,and 7....

22 July 2020 4,616 1 View

Developing Calprotectin Assay for Bovines- extraction buffer selection and centrifuge settings?

Hello, I am trying to adapt the human Calprotectin assay to use in bovines. For background, Calpotectin is a protein released by neutrophils following gastrointestinal inflammation. A couple...

16 March 2020 1,261 3 View

Are there additional practice datasets with code for Multiple-QTL Mapping in R/QTL other than the tutorial on the website?

Multiple-QTL Mapping in R/QTL

20 February 2020 1,469 1 View

Thickest sections possible for slide-mounted IHC of FFPE mouse brain tissue?

I am currently trying to section FFPE mouse brains using a rotary microtome for fluorescence IHC. I'd prefer to collect the sections free floating in buffer, stain them free floating and then...

04 February 2020 6,858 1 View

Antibody recommendation for IHC anti-IL18 staining in mouse brain free floating slices?

Hello, Many papers i look at include the santa cruz M-19 antibody which is now discontinued. I did find a few papers listing the R&D antibody raised against rat (AF521) but I believe this...

02 December 2019 8,578 3 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

18 August 2019 7,599 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View