I tried using an ELISA kit to measure ACE2 in human plasma, however there was no detection. I would like to know if there are more sensitive and easily accessible methods for measuring ACE2?
Amended on 2 October 2019
ACE2 in serum should be determined by utilizing PDMD (protein-direct-micro-sequencing-deciphering) method (please see file; JMBT Alopecia).
Angiotensin-converting enzyme 2/ACE-related carboxypeptidase/Angiotensin-converting enzyme homolog/ACEH/Metalloprotease MPROT15 is present in serum of Gait disorder patient at 6.4 μg/mg of serum protein.
Angiotensin-converting enzyme/CD143/Dipeptidyl carboxypeptidase I/Kininase II/ACE is present in serum of 1y female (with alopecia and biotin deficiency) at 7.5 μg/mg of serum protein.
Since Angiotensin is not hydrolyzed by Human serum biotinidase (please see file; Enk serBIN), and it is absent in Humans. ACE and ACEH may be working in the Amyloid-beta and/or Dynorphin-13 metabolic processes in Humans. Thus ACE2 should be re-named as Amyloid-beta-Dynorphin-converting enzyme.
By the way, Apelin/APJ endogenous ligand is not present in Humans. N-terminal sequence of Apelin-13 is RPRLS-, and it is not hydrolyzed by Human serum biotinidase.
You can try this document. Chapter Measurement of Angiotensin Converting Enzyme 2 Activity in B...
You can also use the radio inhibitor binding assay for human serum ACE. It is a simple, sensitive and specific assay which I find interesting. Just give it a try
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