07 July 2019 4 779 Report

I am looking into getting a plate washer for performing ELISAs to help with consistency and I was wondering if anyone had any reccomendations.

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What can be inferred from ELISA OD values that are higher for wells tested with no capture antibody versus wells with capture antibody?

I am trying to develop and optimize my own indirect sandwhich ELISA by performing cherkerboard titration. I am currently checking different dilutions of the capture antibody against the antigen...

07 August 2019 8,181 8 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

07 August 2019 6,345 4 View

Where could I purchase lypholized Factor V powder so I can use it to create a standard curve in an ELISA?

I am developing my own ELISA for Factor V in mice, however I am having a hard time figuring out how I will be able to run a standard curve. I know people usually use a lypholized powder at a known...

06 July 2019 286 3 View

How do you set up a protocol on a plate reader for a checkerboard titration?

I am using a Gen 5 plate reader system and I am trying to set up a protocol to read a checkerboard titration for an indirect sandwich ELISA I am trying to develop. Would it be correct to define...

06 July 2019 1,652 1 View

What is the recipe to make a carbonate-bicarbonate coating buffer for ELISA?

I will be running an ELISA and I would like to try using a carbonate-bicarbonate coating buffer with a 9.6pH. Does anyone know the correct amounts or percentages of the substances to dissolve into...

06 July 2019 4,613 4 View

What is the correct dilution for a detecting antibody in an ELISA?

I have 0.05 ml of an HRP conjugated antibody that is at a concentration of 1.0 mg/ml. The company reccomends starting at a 1:5,000 dilution, but proper working dilution needs to be optomized....

05 June 2019 2,873 2 View

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