Hi all

I was trying to perform a ELISA, in which I coat the plate with strep, then add in biotin conjugated peptide, then human serum and use anti-human IgG to detect antibody level to the peptide.

I found lots of my human serum samples have high IgG responses to streptavidin (determined by direct ELISA to strep). Is there a way to block these non-specific IgG binding to streptavidin to reduce my ELISA background?

Many thanks

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