I’ve been doing Sandwich Elisa with Greiner Bio-One High Binding Standard ELISA plates (clear polystyren, microlon600 surface treatment, Fisher scientific). It didn’t work well, but we’ve figured that antibodies didn’t have the affinity for the species of interest. Now we’ve changed to different antibodies, provider protocol is very similar to the old one. The main thing that differs in protocol , they suggest using Corning costar 9018 plates (Also clear polystyren, high bind surface treatment??, fisher scientific). They’re also high affinity plates and I cannot find significant enough difference between Corning 9018 plates and greiner bio-one plates. I plan to stick to the greiner plates so there’s less variability between experiments (apparently it’s an issue, according to some reviews). Does anyone has any info on Corning costar 9018 plates? Is there any reason to use them instead of greiner ones?
p.s. I’m analyzing IL-1 beta, 17 Kda, human.
Usually, the different brands are quite similar if you choose the same surface type. If you encounter any problems, it is nearly never the plate to be blamed.
I've crossed compared different Elisa plates and found the grenier high binding plates to be the best and most consistent. I used the costar for screening purposes only.
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