The work of Prof. J. Pogliano on fluorescence microscopic methods of classifying antibacterial mode of action may be of interest to you.
http://biology.ucsd.edu/research/faculty/jpogliano
Agree with Adam's recommendation. Here are two other papers, one describing a genetic approach and the other a classic method, evaluating the effect on macromolecular synthesis:
- Cunningham, Mark L, Bryan P Kwan, Kirk J Nelson, Daniel C Bensen, and Karen J Shaw. “Distinguishing on-Target versus off-Target Activity in Early Antibacterial Drug Discovery Using a Macromolecular Synthesis Assay.” Journal of Biomolecular Screening 18, no. 9 (October 2013): 1018–26. doi:10.1177/1087057113487208.
- Wang, Hao, David Claveau, John P. Vaillancourt, Terry Roemer, and Timothy C. Meredith. “High-Frequency Transposition for Determining Antibacterial Mode of Action.” Nature Chemical Biology 7, no. 10 (October 2011): 720–29. doi:10.1038/nchembio.643.
Dear Anis,
In addition to the answer by Adam, I refer you to similar question rose in ResearchGate. The following link contains the question and about 30 answers.
https://www.researchgate.net/post/Any_suggestions_for_studying_the_antibacterial_mechanism_for_action_of_a_herbal_drug
Hoping this will be helpful,
Rafik
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