Why use special cytoskeletal buffer for superresolution microscopy?

Dear researchers We are preparing for superresolution microscopy. I read some papers where the cells are prepared with the use of a special cytoskeletal buffer (CB) and followed by NaBH4...

01 February 2019 2,574 5 View

Saponin after FA fix in immunostaining?

I want to stain an intracellular protein in 4% FA fixed cultured cells. I have to permeabilise the cells. Triton is to harsh (The ultra structure is too much damaged for EM). I need a milder...

10 November 2018 7,102 5 View

What happened to the Alkaline ethanol and it turned dark brown?

After consulting a colleague I tried to produce sodium ethoxide by mixing NaOH in 96% EtOH. I tried to do so and ended up with a turbid white solution. An earlier question on RG revealed that I...

31 December 2015 3,415 8 View

Sodium ethoxide solution is turned turbid white. Is it normal?

We would like to use sodium ethoxide solution for etching EPON and LR-white EM sections. Today I started making the saturated sodium ethoxide solution. I put 13 g NaOH in 60 ml 96% EtOH. Actually...

10 November 2015 1,696 4 View

How to clean formvar coated Cu or Ni grids for reuse?

11 December 2013 6,018 8 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Magnetismic Gravity in the universe?

MAGNETISMIC GRAVITY What is magnetism? Something magnetic has a + and - charge. If 2 + or - are pushed if + and - are pulled. But what is the main reason for this? Why does magnetism have a pull...

21 February 2021 9,538 3 View