Hello
The Fermentor is in the form of fed batch. In primary and secondary inoculum, growth is normal, but after feeding (about 1/6 of that) about 4 hours later, The fermentor get foamy, the morphology of the cells becomes round and the cells burst and OD reaches to 5 (These events happen in less than 5 minutes). What could be the reason?
Hello Habibeh
Beside of a natural lysis effect, you could check if a bacterial contamination is responsible for an increased lysis in your system
https://pubmed.ncbi.nlm.nih.gov/1892379/
Mostly E.coli-Antibiotic-based systems are not carried out under very strict steril conditions. Autolysis of E.coli is mostly also related to increasing of the pH of the system. Do you regulate Base and Acid in your process? If an alkaline or neutral E.coli Protease is responsible for deformation and autolysis, pH and temperature will be effecting parameters too. Finally: the choice of your anti-foam-reagent can also effect your process. The silicone-based a more effective but also more harmfull to Coli (using emulsified reagent instead of pure can help here) also mechanisms of foam-breaking are different, like Pluronics work differently.
kind regards
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