I want to induce anaemia using phenylhdrazine in mice.. The phenylhadrazine is being injected at 40mg/kg body weight, but everytime i inject the mouse dies in few minutes. Kind of paralyzed and then dies.
an introduction
02 June 2024 520 0 View
I will be grateful if i can get the latest work on the above topic.
17 May 2024 2,509 1 View
I am using the Thermo Scientific DNase I (https://www.thermofisher.com/order/catalog/product/EN0521) to remove genomic DNA from RNA extracts eluted in nuclease free water. The protocol calls for...
30 March 2024 7,100 0 View
My watershed keeps showing there's no overlap with the watershed grid and I have clearly projected it in the same projection as the watershed and source dem. WGS 84, UTM 32. But when try to use...
18 February 2024 1,304 1 View
i would like to ask, wheather autocorrelation desciptors are sub type of 2d or 3d desciptors , they are offered by many platforms to calculate without demon about the differnce between them and...
09 December 2023 8,714 3 View
There's a great app called ChemSketch but only compatible with Windows. I'm looking for similar options for a Mac user. Thnks!
29 November 2023 3,237 2 View
I did an experiment where I evaluated the growth of colonies of a bacteria in different samples. For one of them it was not necessary to do serial dilutions. In this case my dilution factor cannot...
19 November 2023 3,608 8 View
hallo everybody , iam using autodock vina for small molecule docking , however after finalizing everything with the grid the running of autogrid cannot be predeeded and no gld file is created with...
04 November 2023 5,952 2 View
I'm working with a Thermo Scientific GENESYS 10S Series UV-Visible Spectrophotometer. I took readings of a cell culture in two different modes: Absorbance (595 nm) and Conc/Std (Factor = 0.745,...
16 October 2023 2,898 1 View
What is the difference? Does it affect viability? If there is a difference in measurement, is it significant?
15 October 2023 729 2 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View