I want to induce anaemia using phenylhdrazine in mice.. The phenylhadrazine is being injected at 40mg/kg body weight, but everytime i inject the mouse dies in few minutes. Kind of paralyzed and then dies.
Hi everyone, my current Ph.D Project is on "Teachers Engagement in STEM teaching" Please I like to link up with (find) another or any researcher working on Teachers Engagement. I will be grateful...
14 January 2021 8,170 5 View
I have 1851 soil samples data on pH covering a study area of 7482sq.km in northern Ghana and I am using 52 environmental long-term average variables (Relief, Climate, MODIS Reflectances and...
27 December 2020 2,728 6 View
As a novice in the world of research with the aim to publish in a peer-reviewed high-quality journal, you do all you can to get quality work. Sometimes the feedback from reviewers can discourage...
09 September 2020 147 31 View
Have previous publications without DOI with certain journals though they now provide DOIs but don't seems to be ready to link up for the provision of DOIs for previous publications. Any means to...
31 August 2020 9,773 5 View
Does anybody knows if there is a reference style in Mendeley for "Games for Health Journal" ISSN 21617856, 2161783X?
09 July 2020 8,151 1 View
I may need a standard procedure of storage
05 April 2020 163 3 View
Immediately after transforming competent cells with a plasmid ordered from Addgene, I began growing a mini prep culture (5ml) overnight. Next morning, I pelleted the cells and put them in the...
01 February 2020 2,085 2 View
Keynote address
07 September 2019 567 7 View
We are living in the world of fast-paced changes, and thanks to ICT. It is really easing learning, especially among the the young adults. That notwithstanding, adult learning seem not to really...
01 March 2019 522 13 View
I am researching on a chemotherapeutic agent for gliomas which i intend to deliver using nanotechnology.
15 February 2019 9,578 4 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...
28 February 2021 7,882 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...
24 February 2021 3,823 2 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View