Hello, I am planning to do gene knockdown using siRNA. Has anyone used siRNA from Synbio Technologies before? If so, what are your thoughts?
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
For an experimental design ( one experiment and one control group), at least or minimum, how many subjects need to be included in the groups in SEM?
08 January 2024 8,210 3 View
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07 November 2023 4,567 2 View
What I want to ask is this: I have a survey and its analysis. I want to build a mathematical model using these analyses. how can I do it
04 September 2023 9,331 3 View
Superoxide Dismutase Assay Method Activity of SOD has been determined in two ways: 1. Inhibition by the enzyme of an O2- dependent reaction. 2. Pulse radiolytic methods (Rigo et al. 1975) See:...
14 August 2023 3,191 1 View
Hello dear researchers In my study, I have 5 independent variables and 1 dependent variable. In multiple regression analysis, not all my IVs significantly predicted my DV. However, most of my IVs...
27 October 2022 8,623 4 View
I'm using R to run a meta-analysis (using Robust Variance Estimation) of Cohen's d effect sizes. What transformations do I need to do and how do I do them in R? Does anyone have access to online...
13 September 2021 7,239 3 View
Can you help me. I am a student Biology Department. I upload and ran the Native PAGE gel. After 3 hours, even the markers were not visible on the gel. I turned it off and did the painting, but the...
24 June 2021 2,668 2 View
candida albicans biofilm
22 May 2021 9,688 0 View
candidal biofilm
20 May 2021 4,792 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View