How can I prepare disulfiram-copper liposomes using ethanol-based proliposomes method ?

I have a methodology of preparing disulfiram PEGylated liposomes but I am not sure how to attain disulfiram/copper complex in the liposomes.

30 March 2020 4,283 1 View

How can I confirm right colony by PCR?

Hi, I picked up some colonies after electroporation. And I growth those colonies in liquid medium with Erytromycin (200mg/ml). I isolated plasmid after incubation overnight. I did PCR as using...

01 January 2020 9,988 5 View

Significance testing from confidence interval and regression slope?

In a simple regression analysis Seda finds a 95% confidence interval of [-1.4, -0.6] for the unstandardized slope. Is there a statistically significant association between the predictor and the...

07 January 2019 7,050 8 View

SK-MEL 31 cells or B16F10 cells for tyrosinase inhibitor experiments?

Hello Everyone, I have just started to work with animal cell culture studies. I am working on certain novel tyrosinase inhibitors as skin whitening agents. Almost all the literature survey I have...

19 December 2018 8,123 3 View

Why do I see amplification in -RT samples during qPCR?

Hi, During QPCR, I ran non template controls and negative control RT reaction (no RT enzyme using random hexomers and oligodT primers) together with my normal cDNA samples in the same plate. After...

09 July 2018 7,621 4 View

Which steps do you follow after amine synthesis by nitro compounds? and why did the white monomer turn into pink? what can i do not to turn into pink?

02 August 2017 6,211 10 View

Can anyone recommend literature on 'othering'? Is there any socio-pyschological study comparing majority-minority perspectives?

I am specifically asking for socio-psychological studies. I do not like the term "intolerance" since it is politically loaded, but make use of the literature on tolerance, too. I am looking for...

07 September 2015 1,351 6 View

How can I measure the responsivity of a pyroelectric crystal alone?

20 May 2015 5,149 3 View

Which material type is a suitable to design a composite material in ANSYS 14.5?

Thanks in advance for your replies.

28 April 2015 6,558 4 View

Can anyone tell me the lysozyme procedure used for DNA extraction of Lactobacillus sp ?

I need a protocol for lysozyme lysis of Lactobacillus species for DNA extraction from urine and vaginal smear specimens. I have found literatures in which lysozyme lysis is used for Lactobacillus...

11 June 2014 6,886 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View