Has any fly person out here compared in house fly transgenesis Vs commercial services (Best Gene etc.)? How many transgenic per year would make it worth to do it in house, considering equipment acquisition and personnel salary?
Thanks!
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Dear all, I'd like to open here a sort of forum for understanding how the geodesists community is moving in view of the X-band SAR satellite constellation. The new constellation will offer new...
24 January 2021 6,248 0 View
Hi all, I have a collection of Sentinel-2 images and several points within it. In Google Colab, I would like to extract NDVI time series over these points through the whole collection and display...
12 January 2021 4,044 3 View
Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...
09 December 2020 8,832 4 View
Hi everyone, we need some help with a request from a reviewer. Here's the issue: In our study we investigated the impairment in the ability to recognize facial emotions when a facemask is present...
05 November 2020 1,238 18 View
I am reproducing this work on LAMP (isothermal amplification) A new visually improved and sensitive loop mediated isotherm... , but I am a bit confused about the difference between "hydroxy...
31 August 2020 7,289 3 View
Need help in finding the best papers related to the above question. Thank you.
30 August 2020 8,430 12 View
Hi guys! I have a question on a framing effect-like issue. Well, everyone of us has the immediate feeling that there's a huge difference between saying - for instance - "you should respect the...
20 August 2020 7,859 6 View
I am investigating some biocomposites formed by PBAT and tiny particle fillers derived from natural wastes. I have noticed that with a loading of 10%wt. of these fillers, the elongation at break...
28 July 2020 9,367 1 View
Hi, I'm using a 2d CNN. My input size is a square image of 20x20 pixels and my desired output has size 400x3. The most common thing I see is to introduce padding over the convolutional layers...
20 April 2020 2,947 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium
24 February 2021 1,915 1 View