Why Consensus sequence output is as same as refrence genome?

05 March 2023 1 7K Report

After obtaining bam file, I used bcftools to perform vcf, then using bcftools consensus command line included its parameter such as refrence genome, vcf.gz file, the output file with fasta format obtained but its header and appearance is alike the refrence genome fasta format. Is it correct or something is wrong?

Widya Pintaka Bayu Putra

Are your sequence is SNPs panel ?

Badges
Science topic

More Somaye Zareian's questions See All

Outlier detection in labor economics?

I was curious about the possible Machine Learning methods or approaches for outlier (anomaly) detection in labor economics. I tried to search for this matter, but could not find any pertinent...

20 July 2020 1,632 3 View

Vumat "Problem during Compilation"?

Hi There, i am having a problem with inputting my Vumat into Abaqus explicit. I have already inputted many subroutines into Abaqus and got results. However, my recent vumat gives this error...

06 October 2019 4,529 3 View

How can I increase quality of RNA extracted?

RNA sample extracted from the leaf of a medicinal plant with a manual method has the following characteristics, and dissolved in nuclease free water. How can I improve the quality of this sample?...

19 December 2017 1,331 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View