Hello, so i am scaling up a 10 L biorector to 1000 L bioreactor for enzyme production using corncob-based media. The product increased, but there's a lot of excess media left in the product. So, i want to minimize the media impurities before purifying the product. What should i assess regarding this matter? Thank you
The key for fermentation scale up is to keep some key parameters the same or proportional. These important parameters may include mixing times, power to liquid volume ratio, oxygen transfer coefficient etc. The following are some useful article to guide fermentation scale up:
Article Scale-up of Industrial Microbial Processes
https://plygenind.com/how-to-scale-up-fermentation-processes/
If there was no excessive medium at small scale, then the reason for excessive media at scale up is likely due to the unproportional scale up that changed some key parameters.
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