Hello,
I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end)
Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1)
I have done mixing them, and culture results were not that different. They grew well like in normal FBS containing medium.
But I have to do WGS with the cells I grew, so I am suspicious about the result.
Even if the growth rate and cell morphologies are the same, do you think the DNA sequencing results are different when the FBS is mixed with other serum?
Thank you.
Ordinary end point pcr should work well as the structure of the dna has not changed in growing in a mixed medium. There might be differences in gene expression causing changes in the amplification levels in RTPCR between different media but I expect routine pcr to work well.
No problem with DNA sequencing. If you are doing RNAseq or gene expression analysis, then yes it may.
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