Hey guys, I'm looking for an efficient protocol to transfect patient-derived tumor cells with a high rate of transient transfection?

As you know it's quite tricky to get primary cells transfected at a relatively high rate!

31 January 2021 3,580 4 View

What would be the best option to dock a number of peptides to a protein?

I have been using PyRx to dock peptides to a protein. Initially the numbers were small so it was okay. But now I have to dock over 500 peptides and doing so manually would take forever. My...

10 January 2021 3,330 4 View

Does anyone know about vision-guided control of civil structures?

I would like to know more about the following issues: 1. Using images as feedback for the closed-loop control of civil structures 2. Simulating the vision-guided control on the computer without...

03 January 2021 7,566 1 View

How to incorporate a variable in damage model?

15 December 2020 7,284 2 View

Question regarding Primer Designing in qPCR?

Hi Everybody I have not done qPCR, and try to figure out the steps of that. From this regard, I am wondering Should primers designed for mRNA sequence or any other (cDNA, DNA, RNA) in qPCR, if...

07 December 2020 2,203 9 View

Mathematical or computational modelling of protein oxidation?

I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...

17 November 2020 2,340 3 View

Is it possible to still allow me to edit my article?

I made two errors in the references when modifying my article proof. Then I immediately informed the journal. But they prepared the article for publication. And published on the journal site.Is it...

16 November 2020 8,273 6 View

What is the effect of drought and silicon stress on soil pH and EC?

What is the effect of drought and silicon stress on soil pH and EC?

15 November 2020 3,971 5 View

Can FEM model learn from experimental results?

I am looking for a research in which the finite element model was trained based on experimental results.

02 October 2020 9,277 19 View

DLS: How to convert Number%,Intensity% and Volume% to q3,q6 or q0 distributions?

Hello, I am using a Malvern ZetaSizer for my work and as a result in this device, it is possible to get PSD based on Intensity%, Number%, and Volume%. My question is how to convert each of these...

14 September 2020 1,399 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View