Recently I faced a problem while separating 3 species of genus Lethe butterflies (group Woodbrowns -Satyrinae) working along an elevation gradient and different forest habitats in Kedarnath Musk Deer Reserve in the Western Himalaya. Based on wing morphology I separated them into 4 species out of 5 known from the region, but examining of male genitalia revealed only 3 species while the rest were all hybrids!!! Can DNA bar coding help in identification of hybrids and their parents?
Hi
it should be possible. Instead of whole sequencing (if you planning) try to go for established markers in this species for analysis. You may have to use sequence of more than one phylogenetically informative marker/loci
It is possible although you would need to know the species-specific SNPs. This is require sequencing of several individual from each species to find out unique SNPs. Then after that, a simple PCR followed by Sanger sequencing could be useful.
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