Looking expert opinion...
I have collected marine sponge samples and were shadow dried for two weeks. Now the sponge samples are well dried and can be directly powdered by grinding. I would like to study the sponge associated actinobacterial populations (uncultured) from the dried sample rather than fresh sample.
Here comes my doubt,
If we grind and use the sponge powder for metagenomic DNA extraction, does the DNA be damaged/sheared ?
or
can directly use the dried sponge material (without grinding) for metagenomic DNA extraction?
Kindly, some one clarify my doubts.
Thanks in Advance,
Siva
Can't say much about the effect on the microbial community in the dried samples, but I feel using dried samples directly would be a better option if we consider the bias introduced during the sample preparation. Rule of thumb, more the steps involved in the sample prep, more the bias.
Hi,
depending on the used protocol there are bead beatings included. I would use such a protocol as it imporves your lysis. Apart from this it should work and keep in mind that anyhow for metagenome analysis a shotgun library is generated so no worries about fragmentation of your DNA
Sven
Definitely, there is possibility of interference with the DNA quality. Qualitative extraction kit may improve the DNA quality. I suggest you try Mbio products....
- Badges
- Science method