How can you model condensation in ANSYS Fluent 2020?

I have attempted a few tries of recreating a two-phase closed thermosyphon (heat pipe) in Fluent, but am unable to get condensation to work correctly. I have set up water liquid and water vapor as...

25 July 2020 3,125 13 View

Do plant extracts contain essential oil compounds?

So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds. Extracts are formed by immersion of plant matter in a solvent, and are...

12 May 2020 2,119 5 View

How do extract DNA from dried blood spots on Whattman filter papers using Quiagen kit without ATL Buffer?

I have fresh dried blood spots that i need to extract Plasmodium falciparum parasite DNA for analysis of Antimalarial drugs Resistance markers.I will sequence the DNA thus i required high quality...

24 April 2018 6,458 3 View

Is there a scientific calculator or an app to help with calculating alogirithems when computing problems in fundamental computing in Cyber security?

Wondering if there is even mathematical tables to help with word problems?

09 March 2018 198 2 View

Trivalent metallic cations for coagulation?

Does anybody know if using a water soluble salt that generates a trivalent metal cation rather than a divalent cation for sodium alginate coagulation has any impact on the mechanical properties...

16 August 2017 9,390 1 View

Macroinvertebrate people: Have any of you collected any of these large river insects:

Pseudiron centralis, Spinadis simplex, Raptoheptagenia cruentata, Hydroperla fugitans, Amercaenis ridens, Tortopsis puella, Pentagenia vittigera, Harnischia grp. Chironomidae (large river...

06 August 2017 3,906 10 View

I want to potentially undertake a series of focus groups with a team of 15. Has anyone completed a focus group with this many participants?

15 December 2016 7,417 4 View

Having problems with Sigma ReadyMix product# P4600, help?

16 November 2016 578 3 View

Statistical test to use for non-parametric, non matched survey data?

I have conducted two identical surveys, 4 weeks apart, containing Likert scales. Some questions use the scale of "strongly agree" through to "strongly disagree" whilst the others use "very useful"...

03 November 2016 4,402 3 View

How do i get data for mean annual temperatures and precipitation?

I am working on a research on climate change having both economic and climatic variables. the climatic variables at play are temperature and precipitation. how do i source these data for several...

30 August 2016 714 17 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View