Hi Carla,

thermo fisher sales one kit for extracting both molecules at a whole.

https://www.thermofisher.com/fr/en/home/references/ambion-tech-support/rna-isolation/tech-notes/simultaneous-extraction-of-rna-and-dna.html

fred

Hi Carla,

Store the brain tissue with RNA later and then transport with dry ice. Before store in RNA later, chop the tissue with a sterile scalpel blade into thin part which allows RNA later to spread everywhere. I don’t know about aim of your study. But, I suggest trizol is best for bulk preparation of total RNA and genomic DNA. Spin column methods usually good to extract total RNA and DNA from small amount of tissues. I hope this may help u!

Hi, Carla,

I recommend the Quick-DNA/RNA Miniprep Plus Kit(Zymo) for your experiment. You could directly click on the website.

https://www.zymoresearch.com/collections/quick-dna-rna-kits/products/quick-dna-rna-miniprep-plus-kit

Hi, Carla P.D. Fernandes.

All kits available from good companies are ok for the job. I do agree with the other scientists here.

Answering your two questions, I'd say:

- the dissection of the brain tissue will be done abroad and it will need to be transported. What would be the best way to do it? Would dry ice be a good solution?

Answer: For RNA extraction and manipulation, I'd consider use the RNAlater or any other RNA conservative solution (even Trizol) for 24 hours in environmental temperature and then transfer to -80ºC or extract it immediately.

Reminding that RNAlater requests < 0.5cm tissue cut in order to be incorporated in it and then keep your RNA ok to be used lately. Trizol, I do add in the amount indicated by the manufacturer, but after grinding or mechanically disrupting it.

- what would be the best buffer to homogenize the tissue (considering that is a "fatty" tissue and I want to isolate both DNA and RNA) previous to the DNA/RNA isolation and which amount of tissue to use?

The amount of tissue will depend on your kit (usually something between 50-100mg) and the buffer, usually is sterile PBS, but I guess you can use others anyway (reminding to do not add any reagent able to disrupt or degrade your target molecules or complicate the extraction further).

Take a look at this other discussion here.

https://www.researchgate.net/post/RNA_extraction_from_visceral_adipose_tissue

Hope it helps.