How can I connect HC-05 to epoc x ( EEG ) sensor ?

Greetings , my name is Abdul and I have this research on how to move a module car by brain wave . The EEG sensor I have is emotive epoc x , I did the data analysis and am ready to move the car but...

11 January 2021 7,204 1 View

Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Why trans protons and antiperiplanar protons have higher coupling constants in HNMR?

Why in HNMR the coupling constant of trans protons (typically ~17Hz) is higher than the cis protons (typically ~10Hz)? Also, why the antiperiplanar protons in a cyclohexane derivative have higher...

11 June 2020 4,731 4 View

Can I proceed Heck coupling on a compound in the salt form?

Can I do heck coupling reaction on a compound in salt form without turning it into a free base? As for example doing Heck coupling on p-chloropyridiniumchlroide. I don't want to turn my compound...

19 April 2020 5,593 12 View

Is 0.5 ml of DMSO enough to react my (200mg) Ar-Cl with 1.2 equivalent of primary amine using Ullman reaction?

I'm trying to react Ar-CL compound (200mg) with a primary amine (1.2 equivalent), and I've found in the literature that they're using a very low amount of solvents (like 0.5ml) to proceed the...

17 March 2020 1,016 5 View

Can I proceed N-Boc protection for an organic compound which is in the di-phosphate salt form?

My di-phosphate salt compound contains two 3ry amino groups involved in salt formation and a one 2ry amine group that's not involved in salt formation. I wanna proceed N-Boc protection for this...

15 March 2020 945 3 View

My supervisor told me my question and hypothesis are broad and too obvious, what should I do I already finished writing?

So I am writing about hate speech and chose two case studies from Denmark and France. And what I found is that hate speech is heavily influenced by nationalism but my supervisor told it is broad...

03 March 2020 658 3 View

How can I evaporate Methanol from extract by water bath as an alternative of rotavap ?

I have 10 ml methanol extract and unfortunately, my lab is lack a rotavap how can I evaporate this solvent by water bath

16 January 2020 4,897 2 View

HPLC actinomyceyes active compound ?

I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column

29 December 2019 7,578 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View