Is there any phosphates sensor (or total phosphorus) for online monitoring of the lakes?

We need to monitor surface waters in terms of nutrients and algae content. We are building up an on-line in-situ monitoring system for early detection of cyanobacterial blooming and toxicity into...

27 February 2020 9,612 7 View

Would you be interested in hosting a Fulbright Visiting Scholar?

I would like to apply for a Fulbright Visiting Scholarship, but I need a colleague who would like to host me in a U.S. University. My research interests include Special and Inclusive Education,...

08 October 2019 3,089 3 View

Does anyone have any experience with RET immunohistochemistry?

Does anyone have any experience with RET immunohistochemistry? I am interested in staining nerves in skin. Thank you

09 November 2016 7,648 2 View

Is there a way to increase the sensitivity in the "do it yourself/ready-set-go" kits?

19 October 2016 8,696 3 View

What demethylating reagent do you suggest?

I would like to demethylate a substrate which carries one aryl methoxy group and heterocycle moieties. I tried some methods by using HBr and BBr3 but they didn't give any demethylated product. My...

03 August 2016 4,711 19 View

Is anybody working on architectural spaces and/or urban places or neighbourhood projects aiming at well-being of people with dementia condition?

02 August 2016 7,382 8 View

What is the best survey/questionnaire to assess children's perceptions on weapons?

We are conducting a study on primary school children's perceptions of what constitutes a weapon in Greece.

02 July 2015 1,407 2 View

Can I use boron tribromide for the demethylation of a methoxy group on a compound which carries also a primary arylamine?

Do I have to protect the amine?

28 July 2014 3,785 3 View

Imaging extracellular membrane proteins

I want to visualize a protein which will reach the plasma membrane and localize on the extracellular space while performing live cell imaging. Is there a way to have a tag, which will only become...

03 November 2013 582 9 View

When using Autodock Vina is there any way to exclude an allready existed ligand from your protein?

I am currently using an Autodock Vina wizard with PyRx.

10 July 2013 9,534 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View