1. 1.5ml of the bacterial culture was poured into the microcentrifuge tube and then centrifuged at five minutes at 10,000 rpm.
2. the supernatant was discarded and the pellet was left at the bottom of the tube. Another 1.5ml of the culture was poured and the previous steps were repeated until all supernatants were discarded and only the pellet was left at the bottom of the tube.
3. 500µl of distilled water was added into the microcentrifuge tube. The tube was placed on a vortex to mix distilled water and the pellet.
4. After that, the tubes were placed in a heat block at 100°C for ten minutes. The tubes were then quickly placed in ice (-20°C) for five minutes.
5. Then, the tubes were centrifuged at 10,000 rpm for 10 minutes. The supernatants were then transferred into 0.6 ml Eppendorf tubes. The supernatant now contained the bacterial DNA and ready to be analysed.
somewhat variation of the above protocol.
Are you trying to compare your protocol of the inventor of the boiling-centrifuge method?.
Article A simple and rapid method for extracting bacterial DNA from ...
This link is useful
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710730/
regards
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