I want to prepare an aqueous solution of Reserpine to test it's MIC. I've tried to use 8% DMSO as the solvent (which will give me final conc. of 2% DMSO during the assay) but Reserpine precipitates (I dissolved 40mg).
I was able to obtain a solution when using 0.5% v/v acetic acid as the solvent (this gives me 0.125% acetic acid during assay). Some of the bacterial samples are showing growth retardation at this conc. of acetic acid.
I purchased Reserpine from Sigma-Aldrich. So, I don't understand why I am failing to dissolve it in DMSO/Water.
Can anybody suggest me a solvent system which is compatible with Cation Adjusted Mueller Hinton Agar and will not give any antibacterial activity?
In respect to the protocol, you can dissolve about 60 mg/ml of reseprine in clean DMSO. Only 1 mg/ml can be soluble in CAMHB. I guess you need to use less concentrations of your compound for MIC testing. And I would also notice that you can use even 5% DMSO without an affect into cell culture.
DMSO or dimethyl sulfoxide is a good option. Another could be DMF/ dimethyl formamide
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