How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Is BRSV ("bovine respiratory syncytial virus") less susceptible to RNA degradation than SARS-CoV-2 when thawing a sample?

I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?

09 January 2021 4,168 7 View

Can you centrifuge and re-suspend a cDNA sample?

I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...

12 December 2020 3,247 3 View

Is the "Releve" method still standard protocol for plant community sampling and percent cover estimation?

I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...

22 September 2020 5,676 6 View

How to accelerate Langevin dynamics simulations for bead-spring polymer chain models?

I'm running simulations for a bead-spring polymer networks using LAMMPS. I need my simulations to reach biologically relevant timescales of at least a few milliseconds. However each simulation...

14 September 2020 2,954 1 View

Convex Optimization for Deep Learning?

Is there such a thing as convex optimization for large deep learning networks? Is there a way to guarantee convergence? Has anyone looked into this?

06 August 2020 5,384 4 View

What happens in post-secondary education when instructors don't use traditional grades?

I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...

09 June 2020 1,519 6 View

What is the role of journals' reviewers? Should they be absolute judges?

- Ask questions where they are not sure/convinced about a statement/conclusion VS reject a statement/conclusion where they 'think' it is not correct? - Provide suggestions to improve a...

21 May 2020 2,624 7 View

Looking for immunologist or virologist to help with literature-based study for COVID treatment. Can you help?

On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...

29 April 2020 8,098 11 View

Female Soccer - Small sided games - Does training intensity reduce when elite players train with non elite ? or senior players with lower ages groups?

Hi , Im wondering does anyone know of any papers detailing the effects to training intensity of small sided games when the group is of mixed performance levels i.e( professional& semi...

18 December 2019 6,495 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View