I am trying to induce toxicity in hepg2 cells by ccl4.According to paper"HepG2 cells were incubated in medium or treated with the toxic agent (20 mM, 30 mM, or 40 mM CCl4 in 0.05% DMSO".When
i am trying to dilute ccl4 with water or medium or DMSO, instead of mixing they are making two layers.So, how i can dilute CCL4 with water or medium or DMSO.Please give your valuable suggestion.